Understanding Cellular Respiration Processes

May 7, 2025

Lecture Notes: Cellular Respiration

Overview

  • Cellular respiration is a process that oxidizes biological fuels using an inorganic electron acceptor like oxygen.
  • Drives the production of ATP (adenosine triphosphate), which stores chemical energy.
  • Described as metabolic reactions that convert nutrients to ATP, releasing waste products.

Types of Cellular Respiration

  • Aerobic Respiration: Uses oxygen as electron acceptor.
  • Anaerobic Respiration: Uses other molecules than oxygen. Includes fermentation but not considered respiration since no external electron acceptor is involved.

Main Stages of Aerobic Respiration

  1. Glycolysis
    • Occurs in cytosol, splits glucose into two pyruvate molecules.
    • Generates 2 ATP and 2 NADH net gain (4 ATP produced, 2 consumed).
  2. Oxidative Decarboxylation of Pyruvate
    • Converts pyruvate to acetyl-CoA and CO2.
    • Produces NADH in the process.
  3. Citric Acid Cycle (Krebs Cycle)
    • Acetyl-CoA is oxidized in the mitochondrial matrix.
    • Produces NADH, FADH2, and GTP (converted to ATP).
    • Two acetyl-CoA per glucose molecule lead to 6 NADH, 2 FADH2, and 2 ATP.
  4. Oxidative Phosphorylation
    • Involves the electron transport chain and chemiosmosis.
    • Produces the majority of ATP (approximately 30-32 ATP per glucose).

Efficiency of ATP Production

  • Theoretical yield is 38 ATP/glucose, but actual yield is about 29-30 ATP/glucose due to losses like leaky membranes and transport costs.
  • ATP yield varies due to differences in shuttle mechanisms (e.g., glycerol phosphate vs. malate-aspartate shuttle).

Fermentation

  • Occurs when oxygen is absent.
  • Pyruvate undergoes fermentation in cytoplasm, producing waste products like lactic acid or ethanol.
  • Only 2 ATP produced per glucose through substrate-level phosphorylation.

Anaerobic Respiration

  • Uses inorganic electron acceptors other than oxygen (e.g., sulfate, nitrate).
  • Found in organisms in environments void of oxygen, like underwater caves or anoxic soils.

Key Points

  • Aerobic respiration is more efficient than anaerobic, producing more ATP per glucose.
  • The electron transport chain and chemiosmosis are pivotal in ATP synthesis.
  • Different pathways like glycolysis and the citric acid cycle contribute to the overall energy yield.

References

  • Various sources and studies cited within the text provide detailed insights into specific biochemical processes involved in cellular respiration.