Compound Light Microscope: Parts and Operation

Key Components

• Arm and Base

  • Use both to hold and support the microscope securely.

• Oculars (Eyepieces)

  • Adjustable to fit the width of the eyes.
  • Magnification: 10x.

• Revolving Nose Piece

  • Holds multiple objective lenses.
  • Different magnifications available: 10x, 40x, and 100x.

• Stage

  • Holds the slide in place via a clamp.
  • Includes stage moving controls:
    • Moves stage back and forth, right and left.

• Adjustment Dials

  • Coarse Adjustment:
    • Moves stage quickly for rough focusing.
  • Fine Adjustment:
    • Moves stage in small increments for fine focusing.

• Condenser

  • Located under the stage.
  • Controls and focuses light on the specimen.
  • Best position is typically all the way up.

• Iris Diaphragm Lever

  • Located on the condenser.
  • Adjusts the amount of light passing through.

• Light Source

  • Located below the condenser.
  • Front switch to turn on/off.
  • Side dial to adjust brightness.
  • Start with low light intensity.

Operating the Microscope

1. Initial Setup

   • Start with the lowest power objective.
   • Adjust light intensity to low initially.

2. Focusing

   • Use coarse adjustment for initial focus.
   • Fine-tune with fine adjustment.

Cleaning and Storage

• Cleaning

  • Use lens paper to clean oculars and objectives.
  • Lens cleaner may be necessary for removing oil.

• Storage

  • Diminish light intensity and turn off the power.
  • Rotate nose piece to low or no power objective.
  • Lower the stage completely.
  • Wrap power cord around the base/arm.
  • Use dust cover if available.