in this transporation land we're going to measure the rate of water loss by these plants by their transpiration through their stomata on the undersides of these leaves we know that there's little openings by which little pores by which water transpires through and that evaporation through those pores pulls water by bulk flow from the roots all the way to the leaves and so we're going to do is we're going to measure this rate of transpiration by measuring the water loss or the loss of mass over time by putting these plants in two different environmental conditions okay and so in order to do that we're gonna have to bag these plants now these plants are coleus and coleus are shade growing plants and we've grown them in our lab for this purpose okay and I tried to pick plants that had about the same colors and if you notice this one is a little bit lighter now if you notice being little lighter does this is photosynthesize more so we're gonna take this one out but the rest of the remaining plants have about the same color and again we're guessing here a little bit have about the same amount of surface area of leaves they're about the same size so we're gonna use four of these plants okay we're trying to measure the rate of transpiration under three conditions those conditions of course will be 24 hour darkness will do under wind and will do the last 100 24-hour light and so essentially those are our variables that we're going to test in order to see which of those three variables affects transpiration the greatest or the least and keep in mind since transpiration is regulated by our stomata okay those guard cells that open and close this lab is really about measuring how those guard cells work and these we call c3 plants okay any case now if we're gonna measure the rate of water loss we're gonna make sure we have to make sure that the water doesn't evaporate okay from the top of the plant so we're gonna have to bag these okay so I've got four plants one being control that control is gonna be under 12 hour light now we need to bag the plants okay and again again the idea here is to make sure that there's no evaporation okay that occurs here we're trying to make sure the old evaporation or at least in this case transpiration occurs from the top so just taking a very simple Oh one gallon bag okay and I'm just gonna cut the top off because I really don't need at the top and also I did make sure that I really saturated every one of the plants before he started meaning I really water them really good so they're not starting out in a dry or flaccid condition they're all very turgid so I'm putting the plant in a bag okay and you can see what I'm doing here now what I'm gonna do is take a rubber band and I'm gonna gently try to take this extra plastic kind of stuff it in plant and I might lose some lower leaves but that's okay okay so I'm just basically taking this and I'm making sure that all this plastic here is gently pushed into the plant again I do not want to have any of the soil exposed to the air I don't want any evaporation any loss of water would be just due to of course the water through the leaves or is the stomata here so now I'm just gonna put a robe and gently gently I don't want to break the stalk okay that's important so I'm just gonna put this robe and lightly I'm gonna twist once okay and go around the end you want to be careful if I hurt our little our friends and that I think is gonna be tight enough to do what we need to do and we'll do that for the other three so again the whole idea is to just make sure there's no evaporation and we'll do that three more times this being my control of one of the tests that doesn't matter so we'll do that three more times so we have our four bagged plants okay and now we just need to now mash them out alright let's label our plants that's gonna be the control and let's go mass out our control which will be under 12 hour light okay and then let's go measure our other plants that will be under different in different variables of different conditions and so we've got our 24 hour light okay and then we're gonna be label this one the wind 12-hour light with wind and then this one this is gonna be 24-hour darkness and now we need to put them in their different conditions you you you you you