hello guys um i'm gonna be doing biochemistry part two today uh so um bear with me it should not take very long um and it's just like i don't know how you have seen those uh cook shows sorry but that my phone always [Music] goes off whenever i'm doing something earth shaking so um the first part biochemistry part two is the uh the starch testing for starch we have five unknowns in here unknown number five four three uh two uh one so first i'm going to do what is actually a starch i hope you can see that a starch should look like with iodine so iodine is something that tests for starch okay so and then we want to see out of these five unknowns unknown five unknown four three two one which i'll demonstrate that here in a minute which one has starch and then hopefully you will see the end product okay before doing that this is an equipment called micropipette uh it is at 1000 micro there are different sizes uh this is for example measures a 10 microliter this one measures up to 100 microliter and then this one measures up to 1000 microliter so if you want to reduce the amount you go to clockwise yeah i get clockwise and then i want to put oh you know about 500 your lap man he says 500 so let's put 500 of uh starch and right here that is 500 so 500 and i and here is a tip there are different tips here is a tip for that size pipette and then you press it down inside and then this is the tip for 100 and which we are not going to use today and this is the tip for uh 10 uh micro litre okay so what am i gonna do i'll go open up the starch right here mix it up always mix up your solutions before you do none of this stuff you're working on today is a hazardous or anything that i have to worry about um so there are two uh clicks or the first one and the second one you don't want to go to the second all the way down you don't want to do that so you stop where right the blue line is right there you stop so you pump it first and then you go in here by vacuum it comes up and then you put it in starch well right there and then you get rid of the tip that's always they use this in dna a lot uh so whatever we are doing here today is not that earth shaking you tip it off and then you grab a another tip and then you go to the logo solution mix it up a little bit right here and then take that um eye dropper out and then down and then by law of vacuums up and then you dump it in here right there so you should then you would know if in the presence of iodine and starch together that would look like that so now let's get rid of the tip right there i hope you can see everything then i'm gonna go ahead and start doing my unknowns unknown one which is right here and then unknown two and unknown three so i'm not gonna you guys i shake it up i'm not gonna do every single one in here so that oh i i i lost some um thank god these are not hazardous at all so unknown one one and then unknown two i got get rid of the tip on noun two quickly let's do these things um shake it up unknown two get rid of it and now three and so on and so forth okay until unknown five and then i add how much 500 of iodine right here 500 of iodine to each one of these wells 500 here and i'll show you the end results just like as i said i have you watch any cooking channel right here and of course since i'm adding iodine to all of the wells you don't have to change the tip but it's a good practice to change the tip all the time okay so that is for testing the starch right here the next experiment uh that you're supposed to do is for testing simple sugars in this case we have glucose where is my glucose i guess i got rid of it somehow some way it's it's on the other table so my glucose is right here i thought i have two vials so right here is a glucose you can see that okay so oh here it is right there so what am i gonna do uh i put one ml in here now so because we have to boil it uh and according to your lab manual i said use one ml so i i twist this counterclockwise this way you guys you can see that counterclockwise you go to all the way to 1 000 and i think for the rest of the labs we need to do today you need to go to 1000 okay 1986 right here one thousand okay right then you go ahead you grab glucose and here is a test tube for g for glucose and you grab you shake it up shake it up sorry okay you grab one milliliter right your metric system you remember that from metric system so i put one milliliter there or 1000 microliter one milliliter or 1000 microliter i put it there and then my unknowns right get rid of the tip unknown one right i should not close the tip we need them again unknown one it goes into that test loop right here get rid of it unknown two that's right unknown two all right unknown two get rid of it and numb three get rid of it and so on and so forth and five and so on and so forth okay then the benedict solution right here genetic solution it will test for simple sugars so i mix it up very well and then put this aside grab one milliliter put it in the glucose and then again another one milliliter in test tube one and then if you read your lab manual uh test tube two test tube three and test loop four and five and then if you read your lab manual it said that you supposed to uh of course you can mix them up a little bit this is the vortex machine and uh right here i'm mixing them up a little bit right here and wherever you hold your finger that's a maximum um it's gonna go you guys see that it's mixing it up and then here's a glucose of course i've done all five right and then we go to the hot water if you i will bring the camera and show it to you the camera right here it is at 70 celsius let's see if i can show you right here it's boiling water and it is uh where are you can you guys see it it's supposed to be at 70. i i hope you trust me you believe me uh microscope is not the camera is not helping out but it is on seven it's on 70 celsius the temperature of the beacon and at the end the way you're supposed to see things right here this is the starch the results of the starch on noun 1 the starch is on top and then you have unknown 1 2 3 four five which one look like the starch well i would say number four so i would say number four has the storage and then here is for uh the um the benedict solution right we did it i took the bottle so this is the vial that had oh i don't know why you guys cannot see it sorry about that right here are all of the test tubes here is a test tube that had glucose in it and then of course it's been a while uh if you uh look at it it looked like number four at the beginning and number one they had it so i would say number one uh number one or number four i had the glucose in there oh so you are seeing the ceiling now and everything okay so i have to go and adjust this i thought i'm gonna go ahead do it at the end um but okay so let's do the rest of the experiments uh you guys um i better like this machine so i do not lose okay the next experiment um i ask you to um test for oil the best thing to test for oil is paper this grocery bag so here is a vegetable oil i put one drop and you will see the end results here is a vegetable oil here is a distilled water one drop and then here's a pepsi in and a few other things i labeled them for you is a pepsin right here just one quick drop come on pepsi here we go okay and then of course you you went through this experience in life um that what oil look like and water and pepsin and other molecules look like that okay the next one the next one deionized water pepsin so that's for testing fat and then of course you put your unknowns in here and you can test which one has fat or not okay the next one is for testing uh protein so the solution the reagent we use is buret and of course i have albumin and albumin is uh is on my next table i'm gonna get albumin right here is the albumin and i hope you can see that albumin and then it's a protein it's a different type of as a protein and i will add this albumin let's see if i will add it to the a 2 right here so uh how much 500 again uh 1 ml i'm sorry 1 ml so we get rid of this tip i don't know what it was used for so here's albumen i shake it up oh i'm spilling it you guys are lucky well when i was in graduate school i was pretty good you know i was not this clumsy believe it or not but now i guess i'm i'm not being patient enough in life and then of course your unknown one right unknown two unknown three four and five all five of them as you know i'm not gonna do all five of them because i don't want to uh watch me pipette all day and then we'll go to the and results and then uh of course you grab a new tip and then you put buret in every single one of them here's a b red one ml one thousand microliter one ml two one two and three four and five okay just to save the time um right there and then of course your vortex okay so number one number two and so on and so forth that was album so that was for testing the uh protein molecule uh we did uh test for starch simple sugar fat and protein and the next thing i ask you to test for ph we have five unknowns again a noun 1 for ph and i'll move on to the other table unknown two unknown three okay four and five okay then you add i added already a little bit excuse me to these uh the spot plate which we already use i'm going to put these aside okay so i added a little bit that's unknown one two three four five that's for ph now okay then you grab a ph let miss paper ph strip right here do not get it to the tip of your point you have to cut it from right here do not touch that end and you put it in number one you put one in number two oh sorry come on number two you put one in number three number four and number five number five is dry oh sorry number five do you feel better watch so let's do it right now number five uh i'm just i'm not going to measure it you're supposed to measure it i think in your lab manual i just put one drop of oh sorry wrong wrong wrong wrong stuff uh i supposed to graph from the ph good i'm glad i'm making these mistakes so in the lab you will not make those mistakes here's number five and then put this back i hope i just put one little tv maybe i should use how do you like that okay and then of course you compare these colors with this okay so you're comparing these colors which you will see it uh with these um what would be the results okay then the other one i already done it i will show it to you you add your unknown five four three two one to methyl orange and in your lab manual i said uh what color you should get and then phenol phthalene you added another set of the tubes which i will show you will go over there and look at it so let's go over there look at the results okay um i hope um let me take the camera off so i have control over uh what uh i'm showing you guys so here are the stuff that we did and then i think i can unplug maybe oh he doesn't like unplugging [Music] so right here you looked at the um well i'm gonna so you looked at the starch results and then this was for the uh the vegetable oil right right here is a vegetable oil and then i added starch right there i added the egg albumin right there and then this is water right there and pepsin which you know it's a enzyme and then you can see the effect of oil on a paper okay then we went to um out uh the glucose right here is a glucose uh this one the front tube is uh glucose g you can see the g and look at the color so i would say either number one or number four they both have some remedies of the rest look at the blue they haven't changed color originally when i did this about an hour ago it was a number uh before that had pretty much a good coloration same as number one then this is for the uh the benedi the buret test right test for uh proteins and then right here uh the the first one uh you see and then you saw number two and three pretty much look like the alb the albumen right here right here is the a up with a for albumin and b red test for protein molecules and number one number two number three number four number five okay so the ph i did take a this is a ph meter uh i did this last and there are pictures of this in your online stuff so here's a methyl orange what i did with methyl orange right after i did it i took pictures and i again i put one ml of the unknown one two three four five these unknowns maybe i should bring them over and then you guys do not confuse them so this is a ph experiment right on the bottle it says ph 3 4 5 right here four three two and one right here those are the unknowns for ph and this experiment methyl orange is for ph also the um uh phenolphthalein is for ph and then of course i use a ph meter this is a portable ph meter that i used and then you have the pictures of them this is uh this is number one this is number one number two number three number four number five and here are your the unknowns and the unknowns we used for carbohydrates and