chapter 1 the study of Life 1.1 the science of biology by the end of this section you will be able to do the following identify the shared characteristics of the Natural Sciences summarize the steps of the scientific method compare inductive reasoning with deductive reasoning describe the goals of basic science and applied science figure 1.1 this NASA image is a composite of several satellite-based views of earth to make the whole earth image NASA scientists combine observations of different parts of the planet credit NASA gsfc NOAA USGS introduction viewed from space Earth offers no clues about the diversity of life forms that reside there scientists believe that the first forms of life on Earth were microorganisms that existed for billions of years in the ocean before plants and animals appeared the mammals birds and flowers so familiar to us are all relatively recent originating 130 to 250 million years ago the earliest representatives of the genus homo to which we belong have inhabited this planet for only the last 2.5 million years and only in the last 300 000 years have humans started looking like we do today chapter outline 1.1 the science of biology 1.2 themes and concepts of biology figure 1.2 formerly called blue-green algae these a cyanobacteria magnified 300X under a light microscope are some of Earth's oldest life forms these be stromatolites along the shores of Lake Titus in Western Australia are ancient structures formed by layering cyanobacteria in Shallow Waters creditor modification of work by NASA credit B modification of work by Ruth Ellison scale bar data from Matt Russell what is biology in simple terms biology is the study of life this is a very broad definition because the scope of biology is vast biologists may study anything from the microscopic or submicroscopic view of a cell to ecosystems and the Whole Living Planet figure 1.2 listening to the Daily News you will quickly realize how many aspects of biology we discuss every day for example recent news topics include escherichia coli figure 1.3 outbreaks in spinach and salmonella contamination in peanut butter subjects include efforts toward finding a cure for AIDS Alzheimer's disease and cancer on a global scale many researchers are committed to finding ways to protect the planet solve environmental issues and reduce the effects of climate change all of these diverse Endeavors are related to different facets of the discipline of biology figure 1.3 escherichia coli E coli bacteria in this scanning electron micrograph are normal residents of our digestive tracts that Aid in absorbing vitamin K and other nutrients however virulent strains are sometimes responsible for disease outbreaks credit Eric herb digital colorization by Christopher Pooley both of USDA ours emu the process of Science Biology is a science but what exactly is science what does the study a biology share with other scientific disciplines we can define science from the Latin scientia meaning knowledge as knowledge that covers General truths or the operation of general laws especially when Acquired and tested by the scientific method it becomes clear from this definition that applying scientific method plays a major role in science the scientific method is a method of research with defined steps that include experiments and careful observation 8 chapter 1 the study of Life access for free at openstacks.org we will examine scientific method steps in detail later but one of the most important aspects of this method is the testing of hypotheses by means of repeatable experiments a hypothesis is a suggested explanation for an event which one can test although using the scientific method is inherent to science it is inadequate in determining what science is this is because it is relatively easy to apply the scientific method to discipline such as physics and chemistry but when it comes to disciplines like archeology psychology and geology the scientific method becomes less applicable as repeating experiments becomes more difficult these areas of study are still Sciences however consider archeology even though one cannot perform repeatable experiments hypotheses may still be supported for instance an archaeologist can hypothesize that an ancient culture existed based on finding a piece of pottery he or she could make further hypotheses about various characteristics of this culture which could be correct or false through continued support or contradictions from other findings a hypothesis may become a verified Theory a theory is a tested and confirmed explanation for observations or phenomena therefore we may be better off to define science as fields of study that attempt to comprehend the nature of the universe Natural Sciences what would you expect to see in a museum of Natural Sciences frogs plants dinosaur skeletons exhibits about how the brain functions a planetarium gems and minerals maybe all of the above science includes such diverse Fields as astronomy biology Computer Sciences geology logic physics chemistry and Mathematics figure 1.4 however scientists consider those fields of science related to the physical world and its phenomena and processes Natural Sciences thus a Museum of Natural Sciences might contain any of the items listed above figure 1.4 the diversity of scientific Fields includes astronomy biology computer science geology logic physics chemistry mathematics and many other fields credit image editor Flickr there is no complete agreement when it comes to defining what the Natural Sciences include however for some experts the Natural Sciences are astronomy biology chemistry earth science and physics other Scholars choose to divide Natural Sciences into Life Sciences which study living things and include biology and physical sciences which study non-living matter and include astronomy geology physics and chemistry some disciplines such as biophysics and biochemistry build on both life and physical sciences and are interdisciplinary some refer to Natural Sciences as hard science because they rely on the use of 1.1 the science of biology 9 quantitative data social sciences that study society and human behavior are more likely to use qualitative assessments to drive investigations and findings not surprisingly the natural science of biology has many branches or sub-disciplines cell biologists study cell structure and function while biologists who study anatomy investigate the structure of an entire organism those biologists studying physiology however focus on the internal functioning of an organism some areas of biology focus on only particular types of living things for example botanists explore plants while zoologists specialize in animals scientific reasoning one thing is common to all forms of science an ultimate goal to know curiosity and inquiry are the driving forces for the development of science scientists seek to understand the world and the way it operates to do this they use two methods of logical thinking inductive reasoning and deductive reasoning inductive reasoning is a form of logical thinking that uses related observations to arrive at a general conclusion this type of reasoning is common in descriptive science a life scientist such as a biologist makes observations and Records them these data can be qualitative or quantitative and one can supplement the raw data withdrawings pictures photos or videos from many observations the scientist can infer conclusions inductions based on evidence inductive reasoning involves formulating generalizations inferred from careful observation and analyzing a large amount of data brain studies provide an example in this type of research scientists observe many live brains while people are engaged in a specific activity such as viewing images of food the scientist then predicts the part of the brain that lights up during this activity to be the part controlling the response to the selected stimulus in this case images of food excess absorption of radioactive sugar derivatives by active areas of the brain causes the various areas to light up scientists use a scanner to observe the resultant increase in radioactivity then researchers can stimulate that part of the brain to see if similar responses result deductive reasoning or deduction is the type of logic used in hypothesis-based science in deductive reason the pattern of thinking moves in the opposite direction as compared to inductive reasoning deductive reasoning is a form of logical thinking that uses a general principle or law to forecast specific results from those general principles a scientist can extrapolate and predict the specific results that would be valid as long as the general principles are valid studies in climate change can illustrate this type of reasoning for example scientists May predict that if the climate becomes warmer in a particular region then the distribution of plants and animals should change both types of logical thinking are related to the two main Pathways of scientific study descriptive science and hypothesis based science descriptive or Discovery Science which is usually inductive aims to observe explore and discover while hypothesize-based science which is usually deductive begins with a specific question or problem and a potential answer or solution that one can test the boundary between these two forms of study is often blurred and most scientific Endeavors combine both approaches the fuzzy boundary becomes apparent when thinking about how easily observation can lead to specific questions for example a gentleman in the 1940s observed that the bur seeds that stuck to his clothes and his dog's fur had a tiny hook structure on closer inspection he discovered that the Burr's gripping device was more reliable than a zipper he eventually experimented to find the best material that acted similar and produced the hook and loop Fastener popularly known today as Velcro descriptive science and hypothesis-based science are in continuous dialogue the scientific method biologists study the Living World by posing questions about it and seeking science-based responses known as scientific method this approach is common to other Sciences as well the scientific method was used even in ancient times but England Sir Francis Bacon 1561-1626 first documented it figure 1.5 he set up inductive methods for scientific inquiry the scientific method is not used only by biologists researchers from almost all fields of study can apply it as a logical rational problem-solving method 10 chapter 1 the study of Life access for free at openstacks.org figure 1.5 historians credit Sir Francis Bacon 1561-1626 as the first to define the scientific method credit Paul Van Sommer the scientific process typically starts with an observation often a problem to solve that leads to a question let's think about a simple problem that starts with an observation and apply the scientific method to solve the problem one Monday morning a student arrives at class and quickly discovers that the classroom is too warm that is an observation that also describes a problem the classroom is too warm the student then asks a question why is the classroom so warm proposing a hypothesis recall that a hypothesis is a suggested explanation that one can test to solve a problem one can propose several hypotheses for example one hypothesis might be the classroom is warm because no one turned on the air conditioning however there could be other responses to the question and therefore one may propose other hypotheses a second hypothesis might be the classroom is warm because there is a power failure and so the air conditioning doesn't work once one has selected a hypothesis the student can make a prediction a prediction is similar to a hypothesis but it typically has the format if then dot dot dot quote for example the prediction for the first hypothesis might be if the student turns on the air conditioning then the classroom will no longer be too warm testing a hypothesis a valid hypothesis must be testable it should also be falsifiable meaning that experimental results can disprove it importantly science does not claim to prove anything because scientific understandings are always subject to modification with further information this step openness to disproving ideas is what distinguishes Sciences from non-sciences the presence of the supernatural for instance is neither testable nor falsifiable to test a hypothesis a researcher will conduct one or more experiments designed to eliminate one or more of the hypotheses each experiment will have one or more variables and one or more controls a variable is any part of the experiment that can vary or change during the experiment the control group contains every feature of the experimental group except it is not given the manipulation that the researcher hypothesizes therefore if the experimental group's results differ from the control group the difference must be due to the hypothesized manipulation rather than some outside Factor look for the variables and controls in the examples that follow to test the first hypothesis the student would find out if the air conditioning is on if the air conditioning is turned on but does not work there should be another reason and the student should reject this hypothesis to test the second hypothesis the student could check if the lights in the classroom are functional if so there is no power failure and the student should reject this hypothesis the students should test each hypothesis by carrying out appropriate experiments be aware that rejecting one hypothesis does not determine whether or not one can accept the other hypotheses it simply eliminates one hypothesis that is not valid figure 1.6 using the scientific method the student rejects the hypotheses that are inconsistent with experimental data while this warm classroom example is based on observational results other hypotheses and experiments might have clearer controls for instance a student might attend class on Monday and realize she had difficulty concentrating on the lecture 1 1.1 the science of biology 11 observation to explain this occurrence might be when I eat breakfast before class I am better able to pay attention the student could then design an experiment with a control to test this hypothesis in hypothesis-based science researchers predict specific results from a general premise we call this type of reasoning deductive reasoning deduction proceeds from the general to the particular however the reverse of the process is also possible sometimes scientists reach a general conclusion from a number of specific observations we call this type of reasoning inductive reasoning and it proceeds from the particular to the general researchers often use inductive and deductive reasoning in tandem to advance scientific knowledge figure 1.7 visual connection figure 1.6 the scientific method consists of a series of well-defined steps if a hypothesis is not supported by experimental data one can propose a new hypothesis in the example below the scientific method is used to solve an everyday problem match the scientific method steps numbered items with the process of solving the everyday problem lettered items based on the results of the experiment is the hypothesis correct if it is incorrect propose some alternative hypotheses 1. observation a there is something wrong with the electrical outlet 2. question B if something is wrong with the outlet my coffee maker also won't work when plugged into it 12 chapter 1 the study of Life access for free at openstacks.org 3. hypothesis answer Circa my toaster doesn't Toast My Bread 4. prediction D I plug my coffee maker into the outlet 5. experiment e my coffee maker Works 6. result F why doesn't my toaster work visual connection figure 1.7 scientists use two types of reasoning inductive and deductive reasoning to advance scientific knowledge as is the case in this example the conclusion from inductive reasoning can often become the premise for deductive reasoning decide if each of the following is an example of inductive or deductive reasoning 1. all flying birds and insects have wings birds and insects flap their wings as they move through the air therefore Wings enable flight 2. insects generally survive mild Winters better than harsh ones therefore insect pests will become more problematic if global temperatures increase 3. chromosomes the carriers of DNA are distributed evenly between the daughter cells during cell division therefore each daughter cell will have the same chromosome set as the mother cell 4. animals as diverse as humans insects and wolves all exhibit social behavior therefore social behavior must have an evolutionary Advantage the scientific method may seem too rigid and structured it is important to keep in mind that although scientists often follow the sequence there is flexibility sometimes an experiment leads to conclusions that favor a change in approach often and 1.1 the science of biology 13 experiment brings entirely new scientific questions to the puzzle many times science does not operate in a linear fashion instead scientists continually draw inferences and make generalizations finding patterns as their research proceeds scientific reasoning is more complex than the scientific method alone suggests notice too that we can apply the scientific method to solving problems that aren't necessarily scientific in nature two types of science basic science and applied science the scientific Community has been debating for the last few decades about the value of different types of science is it valuable to pursue science for the sake of Simply gaining knowledge or does scientific knowledge only have worth if we can apply it to solving a specific problem or to bettering Our Lives this question focuses on the differences between two types of science basic science and applied science basic science or pure science seeks to expand knowledge regardless of the short-term application of that knowledge it is not focused on developing a product or a service of immediate public or commercial value the immediate goal of basic science is knowledge for knowledge's sake although this does not mean that in the end it may not result in a practical application in contrast applied science or technology aims to use science to solve real-world problems making it possible for example to improve a crop yield find a cure for a particular disease or save animals threatened by a natural disaster figure 1.8 in applied science the problem is usually defined for the researcher figure 1.8 after Hurricane Irma struck the Caribbean in Florida in 2017 thousands of baby squirrels like this one were thrown from their nests thanks to applied science scientists knew how to rehabilitate the squirrel credit Audrey JM 529 Flickr some individuals may perceive applied science as useful and basic science is useless a question these people might pose to a scientist advocating knowledge acquisition would be what for however a careful look at the history of science reveals that basic knowledge has resulted in many remarkable applications of great value many scientists think that a basic understanding of science is necessary before researchers develop an application therefore applied science relies on the results that researchers generate through basic science other scientists think that it is time to move on from basic science in order to find solutions to actual problems both approaches are valid it is true that there are problems that demand immediate attention however scientists would find few Solutions without the help of the wide knowledge Foundation that basic science generates one example of how basic an applied science can work together to solve practical problems occurred after the discovery of DNA structure led to an understanding of the molecular mechanisms governing DNA replication DNA strands unique in every human are in our cells where they provide the instructions necessary for life when DNA replicates it produces new copies of itself shortly before a cell divides understanding DNA replication mechanisms enabled scientists to develop laboratory techniques that researchers now use to identify genetic diseases pinpoint individuals who were at a crime scene and determine paternity without basic science it is unlikely that applied science would exist another example of the link between basic and applied research is the Human Genome Project a study in which researchers analyzed and mapped each human chromosome to determine the precise sequence of DNA subunits in each Gene's exact location the gene is the basic unit of heredity represented by a specific DNA segment that codes for a functional molecule and 14 chapter 1 the study of Life access for free at openstacks.org individuals complete collection of genes as his or her genome researchers have studied other less complex organisms as part of this project in order to gain a better understanding of human chromosomes the Human Genome Project figure 1.9 relied on basic research with simple organisms and later with the human genome an important end goal eventually became using the data for Applied research seeking cures in early diagnoses for genetically related diseases figure 1.9 the Human Genome Project was a 13-year collaborative effort among researchers working in several different science fields researchers completed the project which sequenced the entire Human Genome in 2003 credit the U.S department of energy genome programs HTTP colon genomics.energy.gov HTTP colon slash openstacks.org L genomics underscore gov closing parenthesis while scientists usually carefully plan research efforts in both basic science and applied science note that some discoveries are made by Serendipity that is by means of a fortunate accident or a lucky surprise Scottish biologist Alexander Fleming discovered penicillin when he accidentally left a petri dish of staphylococcus bacteria open an unwanted mold grew on the dish killing the bacteria Fleming's curiosity to investigate the reason behind the bacterial death followed by his experiments led to the discovery of the antibiotic penicillin which is produced by the fungus penicillium even in the highly organized world of science luck when combined with an observant curious mind can lead to unexpected breakthroughs reporting scientific work whether scientific research is basic science or applied science scientists must share their findings in order for other researchers to expand and build upon their discoveries collaboration with other scientists when planning conducting and analyzing results are all important for scientific research for this reason important aspects of a scientist's work are communicating with peers and disseminating results to peers scientists can share results by presenting them at a scientific meeting or conference but this approach can reach only the select few who are present instead most scientists present their results in peer-reviewed manuscripts that are published in scientific journals peer-reviewed manuscripts are scientific papers that a scientist's colleagues or peers review these colleagues are qualified individuals often experts in the same research area who judge whether or not the scientists work is suitable for publication the process of peer review helps to ensure that the research in a scientific paper or Grant proposal is original significant logical and thorough Grant proposals which are requests for research funding are also subject to peer review scientists publish their work so other scientists can reproduce their experiments under similar or different conditions to expand on the findings the experimental results must be consistent with the findings of other scientists a scientific paper is very different from creative writing although creativity is required to design experiments there are fixed guidelines when it comes to presenting scientific results first scientific writing must be brief concise and accurate a scientific paper needs to be succinct but detailed enough to allow peers to reproduce the experiments the scientific paper consists of several specific sections introduction materials and methods results and discussion this structure is sometimes called the imrad format there are usually acknowledgment and reference sections as well as an abstract a concise summary at the beginning of the paper there might be additional sections depending on the type of paper 1.1 the science of biology 15 in the journal where it will be published for example some review papers require an outline the introduction starts with brief but broad background information about what is known in the field a good introduction also gives the rationale of the work it justifies the work carried out and also briefly mentions the end of the paper where the researcher will present the hypothesis or research question driving the research the introduction refers to the published scientific work of others and therefore requires citations following the style of the journal using the work or ideas of others without proper citation is plagiarism the materials and methods section includes a complete and accurate description of the substances the researchers use and the method and techniques they use to gather data the description should be thorough enough to allow another researcher to repeat the experiment and obtain similar results but it does not have to be verbose this section will also include information on how the researchers made measurements and the types of calculations and statistical analyzes they used to examine raw data although the materials and methods section gives an accurate description of the experiments it does not discuss them some journals require a results section followed by a discussion section but it is more common to combine both if the journal does not allow combining both sections the results section simply narrates the findings without any further interpretation the researchers present results with tables or graphs but they do not present duplicate information in the discussion section the researchers will interpret the results describe how variables may be related and attempt to explain the observations it is indispensable to conduct an extensive literature search to put the results in the context of previously published scientific research therefore researchers include proper citations in this section as well finally the conclusion section summarizes the importance of the experimental findings while the scientific paper almost certainly answers one or more scientific questions that the researcher stated any good research should lead to more questions therefore a well-done scientific paper allows the researchers and others to continue and expand on the findings review articles do not follow the imrad format because they do not present original scientific findings or primary literature instead they summarize and comment on findings that were published as primary literature and typically include extensive reference sections 1.2 themes and concepts of biology by the end of this section you will be able to do the following identify and describe the properties of Life describe the levels of organization among living things recognize and interpret a phylogenetic tree list examples of different sub-disciplines in biology biology is the science that studies life but what exactly is life this may sound like a silly question with an obvious response but it is not always easy to Define life for example a branch of biology called virology studies viruses which exhibit some of the characteristics of living entities but lack others although viruses can attack living organisms cause diseases and even reproduce they do not meet the criteria that biologists use to define life consequently virologists are not biologists strictly speaking similarly some biologists study the early molecular Evolution that gave rise to life since the events that preceded life are not biological events these scientists are also excluded from biology in the strict sense of the term from its earliest Beginnings biology has wrestled with three questions what are the shared properties that make something alive once we know something is alive how do we find meaningful levels of organization in its structure finally when faced with the remarkable diversity of Life how do we organize the different kinds of organisms so that we can better understand them as scientists discover new organisms every day biologists continue to seek answers to these and other questions properties of Life all living organisms share several key characteristics or functions order sensitivity or response to the environment reproduction adaptation growth and development regulation homeostasis energy processing and evolution when viewed together these eight characteristics serve to Define life 16 chapter 1 the study of Life access for free at openstacks.org order figure 1.10 a toad represents a highly organized structure consisting of cells tissues organs and organ systems credit ivango Wikimedia Commons organisms are highly organized coordinated structures that consist of one or more cells even very simple single-celled organisms are remarkably complex inside each cell atoms comprise molecules these in turn comprise cell organelles and other cellular inclusions in multicellular organisms figure 1.10 similar cells form tissues tissues in turn collaborate to create organs body structures with a distinct function organs work together to form organ systems sensitivity or response to stimuli figure 1.11 the leaves of this sensitive plant Mimosa putica will instantly droop and fold when touched after a few minutes the plant returns to normal credit Alex Lomas organisms respond to diverse stimuli for example plants can bend toward a source of light climb on fences and walls or respond to touch figure 1.11 even tiny bacteria can move toward or away from chemicals a process called chemotaxis or light phototaxis movement toward a stimulus is a positive response while movement away from a stimulus is a negative response link to learning watch this video HTTP colon slash openstacks.org l slash movement underscore plants closing parenthesis to see how plants respond to a stimulus from opening to light to wrapping a tendril around a branch to capturing prey reproduction single-celled organisms reproduce by first duplicating their DNA and then dividing it equally as the cell prepares to divide to form two new cells multicellular organisms often produce specialized reproductive germline gamete oocyte and sperm cells after fertilization the fusion of an oocyte and a sperm cell a new individual develops when reproduction occurs DNA 1.2 themes and concepts of biology 17 containing genes are passed along to an organism's Offspring these genes ensure that The Offspring will belong to the same species and will have similar characteristics such as size and shape adaptation all living organisms exhibit a fit to their environment biologists refer to this fit as adaptation and it is a consequence of evolution by natural selection which operates in every lineage of reproducing organisms examples of adaptations are diverse and unique from heat resistant archaea that live in boiling hot springs to the tongue length of a nectar-feeding moth that matches the size of the flower from which it feeds all adaptations enhance the reproductive potential of the individuals exhibiting them including their ability to survive to reproduce adaptations are not constant as an environment changes natural selection causes the characteristics of the individuals in a population to track those changes growth and development organisms grow and develop as a result of genes providing Specific Instructions that will direct cellular growth and development this ensures that a species young figure 1.12 will grow up to exhibit many of the same characteristics as its parents figure 1.12 although no two look alike these kittens have inherited genes from both parents and share many of the same characteristics credit Rocky Mountain feline rescue regulation homeostasis even the smallest organisms are complex and require multiple regulatory mechanisms to coordinate internal functions respond to stimuli and cope with environmental stresses two examples of internal functions regulated in an organism are nutrient transport and blood flow organs groups of tissues working together perform specific functions such as carrying oxygen throughout the body removing wastes delivering nutrients to every cell and cooling the body figure 1.13 polar bears ursus maritimus and other mammals living in ice-covered regions maintain their body temperature by generating heat and reducing heat loss through thick fur in a dense layer of fat under their skin credit Longhorn Dave flicker in order to function properly cells require appropriate conditions such as proper temperature pH and appropriate concentration of diverse chemicals these conditions May however change from one moment to the next organisms are able to 18 chapter 1. the study of Life access for free at openstacks.org maintain internal conditions within a narrow range almost constantly despite environmental changes through homeostasis literally steady state for example an organism needs to regulate body temperature through the thermoregulation process organisms that live in cold climates such as the polar bear figure 1.13 have body structures that help them withstand low temperatures and conserve body heat structures that Aid in this type of insulation include fur feathers blubber and fat in hot climates organisms have methods such as perspiration in humans or panting in dogs that help them to shed excess body heat energy processing figure 1.14 the California condor gymnog chips californianus uses chemical energy derived from food to power flight California Condors are an endangered species this bird has a wing tag that helps biologists identify the individual credit Pacific southwest region U.S fish and wildlife service all organisms use a source of energy for their metabolic activities some organisms capture energy from the Sun and convert it into chemical energy in food others use chemical energy and molecules they take in as food figure 1.14 Evolution the diversity of life on Earth as a result of mutations or random changes in hereditary material over time these mutations allow the possibility for organisms to adapt to a changing environment an organism that evolves characteristics fit for the environment will have greater reproductive success subject to the forces of natural selection levels of organization of living things living things are highly organized and structured following a hierarchy that we can examine on a scale from small to large the atom is the smallest and most fundamental unit of matter it consists of a nucleus surrounded by electrons atoms form molecules a molecule is a chemical structure consisting of at least two atoms held together by one or more chemical bonds many molecules that are biologically important are macromolecules large molecules that are typically formed by polymerization a polymer is a large molecule that is made by combining smaller units called monomers which are simpler than macromolecules an example of a macromolecule is deoxyribonucleic acid DNA figure 1.15 which contains the instructions for the structure and functioning of all living organisms 1.2 themes and concepts of biology 19 figure 1.15 all molecules including this DNA molecule are comprised of atoms credit brian0918 Wikimedia Commons linked to learning watch this video HTTP colon slash openstacks.org L rotating underscore DNA closing parenthesis that animates the three-dimensional structure of the DNA molecule in figure 1.15 some cells contain Aggregates of macromolecules surrounded by membranes we call these organelles organelles are small structures that exist within cells examples of organelles include mitochondria and chloroplasts which carry out indispensable functions mitochondria produce energy to power the cell while chloroplasts enable green plants to utilize the energy and sunlight to make sugars all living things are made of cells the cell itself is the smallest fundamental unit of structure and function in living organisms this requirement is why scientists do not consider viruses living they are not made of cells to make new viruses they have to invade and hijack the reproductive mechanism of a living cell only then can they obtain the materials they need to reproduce some organisms consist of a single cell and others are multicellular scientists classify cells as prokaryotic or eukaryotic prokaryotes are single-celled or Colonial organisms that do not have membrane-bound nuclei in contrast the cells of eukaryotes do have membrane-bound organelles and a membrane-bound nucleus in larger organisms cells combine to make tissues which are groups of similar cells carrying out similar or related functions organs are collections of tissues grouped together performing a common function organs are present not only in animals but also in Plants an organ system is a higher level of organization that consists of functionally related organs mammals have many organ systems for instance the circulatory system transports blood through the body and to end from the lungs it includes organs such as the heart and blood vessels organisms are individual living entities for example each tree in a forest is an organism single-celled prokaryotes and single-celled eukaryotes are also organisms which biologists typically call microorganisms 20 chapter 1 the study of Life access for free at openstacks.org biologists collectively call all the individuals of a species living within a specific area population for example a forest may include many pine trees which represent the population of pine trees in this Forest different populations may live in the same specific area for example the forest with the pine trees includes populations of flowering plants insects and microbial populations a community is the sum of populations inhabiting a particular area for instance all of the trees flowers insects and other populations in a forest form the Forest community the forest itself is an ecosystem an ecosystem consists of all the living things in a particular area together with the abiotic non-living parts of that environment such as nitrogen in the soil or Rainwater at the highest level of organization figure 1.16 the biosphere is the collection of all ecosystems and it represents the zones of life on Earth it includes land water and even the atmosphere to a certain extent visual connection figure 1.16 shows the biological levels of organization of living things from a single organelle to the entire biosphere living organisms are parts of a highly structured hierarchy credit organelles modification of work by Umberto salvenin credit cells modification of work by Bruce Wetzel Harry Shafer National Cancer Institute credit tissues modification of work by kilbad Fama clamosa Michael hagstrom credit organs modification of work by Mariana Ruiz virial credit organisms modification of work by Crystal flicker credit ecosystems modification of work by U.S fish and wildlife Service headquarters credit biosphere modification of work by NASA which of the following statements is false a tissues exist within organs which exist within organ systems B communities exist within populations which exist within ecosystems C organelles exist within cells which exist within tissues D communities exist within ecosystems which exist in the biosphere the diversity of Life the fact that biology as a science has such a broad scope has to do with the tremendous diversity of life on Earth the source of this diversity is evolution the process of gradual change in a population or species over time evolutionary biologists study the 1.2 themes and concepts of biology 21 evolution of living things in everything from the microscopic world to ecosystems a phylogenetic tree figure 1.17 can summarize the evolution of various life forms on Earth it is a diagram showing the evolutionary relationships among biological species based on similarities and differences in genetic or physical traits or both nodes and branches comprise a phylogenetic tree the internal nodes represent ancestors and are points in evolution when based on scientific evidence researchers believe an ancestor has diverged to form two new species the length of each branch is proportional to the time elapsed since the split figure 1.17 microbiologist Carl wos constructed this phylogenetic tree using data that he obtained from sequencing ribosomal RNA genes the tree shows the separation of living organisms into three domains bacteria Archaea and Eukarya bacteria and Archaea are prokaryotes single-celled organisms lacking intracellular organelles credit Eric Gaba NASA astrobiology Institute Evolution connection Carl wos and the phylogenetic tree in the past biologists grouped living organisms into five kingdoms animals plants fungi protists and bacteria they based the organizational scheme mainly on physical features as opposed to physiology biochemistry or molecular biology all of which modern systematics use American microbiologist Carl wos's pioneering work in the early 1970s has shown however that life on Earth has evolved along three lineages now called domains bacteria Archaea and Eukarya the first two are prokaryotic cells with microbes that lack membrane enclosed nuclei and organelles the third domain contains the eukaryotes and includes unicellular microorganisms protists together with the three remaining kingdoms fungi plants and animals woes defined archaea as a new domain and this resulted in a new taxonomic tree figure 1.17 many organisms belonging to the archaea domain live under extreme conditions and are called extremophiles to construct his tree wosu's genetic relationships rather than similarities based on morphology shape wos constructed his tree from universally distributed comparative Gene sequencing that are present in every organism and conserved meaning that these genes have remained essentially unchanged throughout Evolution wos's approach was revolutionary because comparing physical features are insufficient to differentiate between the prokaryotes that appear fairly similar in spite of their tremendous biochemical diversity and genetic variability figure 1.18 comparing homologous DNA and RNA sequences provided woes with a sensitive device that revealed the extensive variability of prokaryotes and which Justified separating the prokaryotes into two domains bacteria and archaea 22 chapter 1 the study of Life access for free at openstacks.org figure 1.18 these images represent different domains the bacteria in this micrograph belong to domain bacteria while the B extremophiles not visible living in this hot vent belong to domain Archaea both the sea sunflower and d lion are part of domain Eukarya creditor modification of work by Drew March credit B modification of work by Steve gervatson credit C modification of work by Michael irigi credit D modification of work by leshik lozinski branches of biological study the scope of biology is Broad and therefore contains many branches and sub-disciplines biologists May pursue one of those sub-disciplines and work in a more Focused Field for instance molecular biology and biochemistry study biological processes at the molecular and chemical level including interactions among molecules such as DNA RNA and proteins as well as the way they are regulated microbiology the study of microorganisms is the study of the structure and function of single-celled organisms it is quite a broad Branch itself and depending on the subject of study there are also microbial physiologists ecologists and geneticists among others Career Connection forensic scientist forensic science is the application of science to answer questions related to the law biologists as well as chemists and biochemists can be forensic scientists forensic scientists provide scientific evidence for use in courts and their job involves examining Trace materials associated with crimes interest in forensic science has increased in the last few years possibly because of popular television shows that feature forensic scientists on the job also developing molecular techniques and establishing DNA databases have expanded the types of work that forensic scientists can do their job activities are primarily related to crimes against people such as murder rape and assault their work involves analyzing samples such as hair blood and other body fluids and also processing DNA figure 1.19 found in many different environments and materials forensic scientists also analyze other biological evidence left at crime scenes such as insect larva or pollen grains students who want to pursue careers in forensic science will most likely have to take chemistry and biology courses as well as some intensive math courses 1.2 themes and concepts of biology 23 figure 1.19 this forensic scientist Works in a DNA extraction room at the U.S army criminal investigation laboratory at Fort Gilliam ga credit United States Army Sid command public affairs another field of biological study neurobiology studies the biology of the nervous system and although it is a branch of biology it is also an interdisciplinary field of study known as neuroscience because of its interdisciplinary nature this sub-discipline studies different nervous system functions using molecular cellular developmental medical and computational approaches figure 1.20 researchers work on Excavating dinosaur fossils at a site in castelon Spain credit Mario Modesto paleontology another branch of biology uses fossils to study life's history figure 1.20 zoology and botany are the study of animals and plants respectively biologists can also specialize as biotechnologists ecologists or physiologists to name just a few areas this is just a small sample of the many fields that biologists can pursue biology is the culmination of the achievements of the Natural Sciences from their Inception to today excitingly it is the Cradle of emerging Sciences such as the biology of brain activity genetic engineering of custom organisms and the biology of evolution that uses the laboratory tools of molecular biology to retrace the earliest stages of life on Earth a scan of news headlines whether reporting on immunizations a newly discovered species Sports doping or a genetically modified food demonstrates the way biology is active and and important to our everyday world