Overview
This transcript outlines the step-by-step procedure to prepare platelet-rich plasma using the Pure 2 platelet concentrating system, including setup, centrifugation, and PRP collection.
Materials and Preparation
- Pure 2 separator device and counterbalance device
- Sterile syringes: 30 mL and 12 mL
- Citrate anticoagulant: 3 mL per 30 mL blood draw
- Sterile alcohol swabs; clear non-vented caps
- Centrifuge with rotor and Pure 2 IFU protocol settings
Procedure Summary Table
| Step | Action | Details | Key Checks |
|---|
| 1 | Prepare separator port | Remove Red Cap; swab with sterile alcohol before and after access | Maintain sterility |
| 2 | Prepare syringe | Fill 30 mL syringe with 3 mL citrate anticoagulant | Correct anticoagulant volume |
| 3 | Draw blood | Draw 27 mL patient blood to reach 30 mL total | Gentle handling |
| 4 | Load separator | Attach 30 mL syringe to needleless port; slowly inject anticoagulated blood | Slow injection |
| 5 | Counterbalance | Use counterbalance device with equal volume | Equal volume on both sides |
| 6 | First centrifugation | Place devices opposite in rotor; follow Pure 2 IFU protocol; start | Secure lid; correct settings |
| 7 | Aspirate PPS | Attach sterile syringe to top port; aspirate platelet plasma suspension | PPS above red blood cells |
| 8 | Transfer to concentrator | Hold concentrator upright; transfer PPS through bottom port | Steady transfer |
| 9 | Cap before spin | Attach clear non-vented cap to bottom port; do same for counterbalance device | Mandatory step |
| 10 | Second centrifugation | Place devices opposite; follow IFU settings; start | Equal volume; secure caps |
| 11 | Remove excess plasma | Attach syringe to needleless port; aspirate plasma; leave 3–4 mL or desired amount | Buffy coat visible at bottom |
| 12 | Resuspend platelets | Rock and swirl until Buffy coat fully resuspends into plasma | No visible Buffy coat remains |
| 13 | Collect PRP | Attach 12 mL syringe to needleless port; tilt device; aspirate PRP | Smooth aspiration |
Detailed Steps
- Port preparation and access
- Remove Red Cap from needleless valve port of separator.
- Swab port with sterile alcohol before and after syringe access.
- Anticoagulation and blood draw
- Fill 30 mL sterile syringe with 3 mL citrate anticoagulant.
- Draw 27 mL blood to reach 30 mL total volume in the syringe.
- Loading and first centrifugation
- Attach 30 mL blood syringe to separator needleless port; inject slowly.
- Counterbalance using equal volume device; place opposite in rotor.
- Follow Pure 2 IFU protocol settings; close lid; press start.
- After spin, platelet plasma suspension is above red blood cell layer.
- Transfer to concentrator and mandatory capping
- Attach sterile syringe to top port; aspirate platelet plasma suspension.
- Hold concentrator upright; transfer suspension through bottom port.
- Attach clear non-vented cap to bottom port before centrifugation.
- Cap bottom port of counterbalance device as well.
- Second centrifugation and concentration
- Counterbalance with equal volume; place opposite in rotor.
- Follow Pure 2 IFU protocol settings; close lid; start centrifuge.
- After spin, platelet Buffy coat is visible at device bottom.
- Plasma removal and resuspension
- Attach sterile syringe to needleless port; aspirate plasma.
- Leave 3–4 mL plasma or desired amount in device.
- Rock and swirl until all platelets are resuspended into plasma.
- Ensure no Buffy coat remains visible at the bottom.
- PRP collection
- Attach 12 mL syringe to needleless port.
- Tilt device to aspirate platelet-rich plasma smoothly.
Key Terms & Definitions
- Platelet plasma suspension (PPS): Plasma layer containing platelets above the red blood cell layer after the first spin.
- Buffy coat: Concentrated layer rich in platelets located at the bottom after the second spin.
- PRP (Platelet-Rich Plasma): Final platelet-enriched plasma collected after resuspension.
- IFU: Instructions for Use; protocol settings specific to the Pure 2 system.
Action Items / Next Steps
- Verify centrifuge protocol settings per Pure 2 IFU before each spin.
- Always attach clear non-vented cap to bottom port before the second spin.
- Ensure equal volume counterbalance placement at opposite rotor positions.
- Confirm complete resuspension of Buffy coat before PRP aspiration.