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Overview of Cells and Microscopy

May 27, 2025

Chapter 4: Cells Overview

Introduction to Cells

  • Cells are the smallest unit of life.
  • All living things are made of one or more cells.
  • Types of Organisms:
    • Unicellular: Single-celled organisms.
    • Multicellular: Organisms like humans made of multiple cells.
  • Cells with common functions form tissues, which combine to form organs, and organs make up organ systems.

Microscopy

  • Microscopes: Used to view cells too small for the naked eye.
  • Magnification & Resolution:
    • Magnification depends on the types of lenses.
    • Resolution distinguishes two adjacent structures as separate.
  • Types of Microscopes:
    • Light Microscopes: Use visible light.
    • Electron Microscopes: Use electrons for higher resolution.
  • Staining: Used to view transparent cells, but usually kills them.
  • Phase Contrast Microscopy: Allows viewing of live, unstained specimens.

Microscopy Types for Cells

  • Transmission Electron Microscope (TEM): Provides detail within cells.
  • Scanning Electron Microscope (SEM): Provides 3D exterior views.

Cell Theory

  • Tenets:
    1. Cells are the basic units of life.
    2. All living things are made of cells.
    3. All cells come from pre-existing cells.
  • Cytotechnologists: Study cells to detect abnormalities, such as HPV infections.

Basic Cell Components

  • Common to All Cells:
    • Plasma membrane.
    • Cytoplasm (includes cytosol and structures).
    • DNA.
    • Ribosomes.

Prokaryotic Cells

  • Characteristics:
    • No nucleus or membrane-bound organelles.
    • DNA is in the nucleoid.
    • Often have cell walls made of peptidoglycan.
    • Domains: Bacteria and Archaea.
  • Structures:
    • Ribosomes, cell membrane, and cell wall.
    • May have flagella, pili, and capsules.
  • Size: Prokaryotes are 10-100 times smaller than eukaryotic cells.

Cell Size and Surface Area to Volume Ratio

  • Importance of Efficient Transport:
    • Smaller cells are more efficient due to a higher surface area to volume ratio.
    • Large cells have more difficulty transporting materials.
  • Adaptations in Large Cells:
    • Increase surface area through invaginations or becoming flat.
  • Prokaryotic vs. Eukaryotic Cells:
    • Prokaryotic cells lack complex structures that aid in transport.

Conclusion of Part 1

  • Prokaryotic cells discussed; eukaryotic cells will be covered in Part 2.