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Overview of Cells and Microscopy
May 27, 2025
Chapter 4: Cells Overview
Introduction to Cells
Cells are the smallest unit of life.
All living things are made of one or more cells.
Types of Organisms:
Unicellular:
Single-celled organisms.
Multicellular:
Organisms like humans made of multiple cells.
Cells with common functions form tissues, which combine to form organs, and organs make up organ systems.
Microscopy
Microscopes:
Used to view cells too small for the naked eye.
Magnification & Resolution:
Magnification depends on the types of lenses.
Resolution distinguishes two adjacent structures as separate.
Types of Microscopes:
Light Microscopes:
Use visible light.
Electron Microscopes:
Use electrons for higher resolution.
Staining:
Used to view transparent cells, but usually kills them.
Phase Contrast Microscopy:
Allows viewing of live, unstained specimens.
Microscopy Types for Cells
Transmission Electron Microscope (TEM):
Provides detail within cells.
Scanning Electron Microscope (SEM):
Provides 3D exterior views.
Cell Theory
Tenets:
Cells are the basic units of life.
All living things are made of cells.
All cells come from pre-existing cells.
Cytotechnologists:
Study cells to detect abnormalities, such as HPV infections.
Basic Cell Components
Common to All Cells:
Plasma membrane.
Cytoplasm (includes cytosol and structures).
DNA.
Ribosomes.
Prokaryotic Cells
Characteristics:
No nucleus or membrane-bound organelles.
DNA is in the nucleoid.
Often have cell walls made of peptidoglycan.
Domains: Bacteria and Archaea.
Structures:
Ribosomes, cell membrane, and cell wall.
May have flagella, pili, and capsules.
Size:
Prokaryotes are 10-100 times smaller than eukaryotic cells.
Cell Size and Surface Area to Volume Ratio
Importance of Efficient Transport:
Smaller cells are more efficient due to a higher surface area to volume ratio.
Large cells have more difficulty transporting materials.
Adaptations in Large Cells:
Increase surface area through invaginations or becoming flat.
Prokaryotic vs. Eukaryotic Cells:
Prokaryotic cells lack complex structures that aid in transport.
Conclusion of Part 1
Prokaryotic cells discussed; eukaryotic cells will be covered in Part 2.
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