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Osmosis - Sideroblastic Anemia

Apr 21, 2025

Lecture Notes: Sideroblastic Anemia

Overview

  • Sideroblastic Anemia: A type of blood disorder characterized by an accumulation of iron in red blood cells (RBCs), leading to immature and dysfunctional RBCs.
  • Mechanism: RBCs are unable to incorporate iron into hemoglobin, impairing their ability to transport oxygen.

Hemoglobin and Heme Synthesis

  • Hemoglobin: Composed of hemes and globins, responsible for carrying oxygen.
    • Globin Subunits: Typically two alpha and two beta, each with a heme group.
    • Heme: Large molecule made of four pyrrole subunits forming a porphyrin ring, containing ionically bonded iron (Fe2+).
    • Oxygen Binding: Each hemoglobin can carry four oxygen molecules.
  • Heme Synthesis: Involves mitochondria and cytosol and multiple enzymes.
    • Begins in mitochondria: Succinyl-CoA + Glycine -> Delta-aminolevulinic acid (ALA) via Delta ALA synthase, requiring Vitamin B6.
    • In cytosol: Delta-ALA -> Porphobilinogen (PBG) via Delta-ALA dehydratase.
    • Condensation: Four PBG -> Hydroxymethylbilane aided by Porphobilinogen deaminase.
    • Further transformations: Hydroxymethylbilane -> Uroporphyrinogen III -> Coproporphyrinogen III in mitochondria.
    • Iron incorporation: Iron added to Protoporphyrin IX by ferrochelatase.

Pathophysiology of Sideroblastic Anemia

  • Defective Protoporphyrin Synthesis: Leads to impaired iron incorporation to form heme.
  • Types:
    • Congenital: Often X-linked, affecting mostly males due to ALAS2 gene mutations, impairing delta ALA synthase.
    • Acquired:
      • Excessive alcohol use causing mitochondrial damage.
      • Pyridoxine (Vitamin B6) deficiency.
      • Lead poisoning, inhibiting crucial enzymes like Delta-ALA dehydratase and ferrochelatase.
  • Characteristic Features:
    • Basophilic stippling in RBCs due to ribosome accumulation.
    • Ringed sideroblasts in bone marrow.

Clinical Presentation and Diagnosis

  • Symptoms: Fatigue, potential heart disease, liver damage, enlarged spleen, kidney failure, and diarrhea due to iron overload.
  • Diagnosis:
    • Clinical Evaluation: Similar to hemochromatosis.
    • Laboratory Findings:
      • Complete blood count: Normal or low mean corpuscular volume.
      • Peripheral blood smear: Basophilic stippling and Pappenheimer bodies.
      • Iron studies: High serum iron, increased ferritin levels, decreased total iron binding capacity.

Treatment

  • Removal of Toxins: Important in acquired cases (e.g., alcohol, lead).
  • Nutritional Support: Administration of pyridoxine, thiamine, and folic acid.
  • Iron Overload Management:
    • Therapeutic phlebotomy or chelation therapy (deferoxamine).
  • Severe Cases: May require bone marrow or liver transplant.

Recap

  • Cause: Either congenital abnormalities or acquired conditions lead to a failure in iron incorporation for heme production.
  • Impact: Iron overload damages organs, and lack of functional heme results in anemia and fatigue.
  • Management: Focus on toxin removal and vitamin supplementation to improve heme synthesis.