🩸

Phlebotomy Order of Draw Guide

Mar 13, 2025

Flashcard/Quiz Key Points:

I. Order of Draw:

  • Purpose: Prevent cross-contamination of additives.
  • Sequence: Yellow, Light Blue, Red, Green, Lavender, Gray. (Note: Some institutions may have variations or omissions)

II. Tube Types & Additives:

  • Yellow (SPS): Anticoagulant: SPS; Tests: Blood Cultures; Specimen: Whole Blood.
  • Light Blue (Sodium Citrate): Anticoagulant: Sodium Citrate; Tests: PT, PTT, aPTT, INR; Specimen: Plasma.
  • Red (No Additive): Anticoagulant: None; Tests: Serum chemistry tests (e.g., cholesterol, blood urea nitrogen); Specimen: Serum.
  • Green (Heparin): Anticoagulant: Heparin; Tests: BMP, electrolytes, LFTs; Specimen: Plasma.
  • Lavender (EDTA): Anticoagulant: EDTA; Tests: CBC (RBC, WBC, Hgb, Hct); Specimen: Whole Blood.
  • Gray (Potassium Oxalate/Sodium Fluoride): Anticoagulant: Potassium Oxalate/Sodium Fluoride; Tests: Fasting Blood Sugar, Glucose; Specimen: Plasma (fasting sample recommended).

III. Inversions:

  • The number of inversions varies slightly depending on tube type and manufacturer instructions; always check the label.
  • General Guidelines: 8-10 inversions for most tubes with anticoagulants. Fewer for some.

IV. Mnemonic Device (Example):

  • **Yellow (Young): Blood Cultures
  • **Light Blue (Lovely): Coagulation
  • **Red (Rabbits): Serum
  • **Green (Go to): Heparin
  • **Lavender (Laughing): CBC
  • **Gray (Girls): Glucose
  • Mnemonic Phrase: Young Lovely Rabbits Go To Laughing Girls

V. Important Considerations:

  • Reflux: The backflow of additives from one tube into another, causing contamination.
  • Always follow your facility's specific protocols and procedures.

Use these key points to easily create effective flashcards and quizzes within Coconote to reinforce your learning. Good luck with your studies!