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Protein Structure and Function

Sep 10, 2025

Overview

This lecture covers the structure, composition, and function of proteins, detailing how amino acids form proteins and their roles in the human body.

Protein Structure and Composition

  • Proteins are macromolecules composed of carbon, hydrogen, oxygen, and nitrogen atoms.
  • The basic subunit (monomer) of a protein is the amino acid.
  • Each amino acid contains an amine group (NHâ‚‚), a carboxyl group (COOH), and a unique functional group.
  • Proteins are formed when amino acids join via peptide bonds through dehydration synthesis (removal of water).
  • A long chain of amino acids linked together is called a polypeptide.

Amino Acids

  • There are 20 different amino acids found in proteins.
  • The functional group attached to each amino acid determines its unique properties and behavior.
  • Essential amino acids cannot be synthesized by the body and must be obtained from the diet.
  • Functional groups can be hydrophobic (repel water), hydrophilic (attract water), acidic, basic, positively charged, or negatively charged.

Protein Folding and Function

  • Functional groups cause polypeptide chains to fold into specific 3D shapes (quaternary structures).
  • The folding is influenced by attraction or repulsion between different functional groups (e.g., positive vs. negative, hydrophobic vs. hydrophilic).
  • The 3D structure of a protein determines its function in the body.
  • Proteins serve as enzymes, receptors, transporters, and structural components.
  • Mutations that change even a single amino acid can alter protein folding and disrupt its function.

Key Terms & Definitions

  • Protein — A large, complex molecule made of one or more folded polypeptide chains.
  • Amino acid — The monomer unit of proteins, containing an amine group, carboxyl group, and a side functional group.
  • Peptide bond — The covalent bond formed between two amino acids during protein synthesis.
  • Polypeptide — A chain of amino acids linked by peptide bonds.
  • Essential amino acids — Amino acids that must be acquired through diet as the body cannot synthesize them.
  • Functional group — The variable side group that gives each amino acid its unique properties.

Action Items / Next Steps

  • Review the structure of amino acids and how peptide bonds form.
  • Memorize the essential amino acids.
  • Prepare for questions on how protein structure relates to function and effects of mutations.