foreign we're going to do the whole plant transpiration lab where we're looking at the effect of different conditions on the transpiration rate so we've got our plants here we're going to be using impatience but you can use any plant that you want whatever's available at your local nursery and so what we want to do is we want to take the plants out of the container that it comes in so you want to take the root ball out and these plants have recently been watered so there's a good water source in there and we're going to put the whole root ball into our plastic bag sometimes it helps to kind of Squish it down so it stands upright and we are going to tie the bag around the root ball but we're also going to label it with these little tags so that we know the initial mass and the mass as we go for each time that we record our data so the next three class periods we are going to measure how much the whole plant weighs so we're going to mask it today we're going to mask it next class and then the following class as well tying it off you want to tie it at the base of the plant to make sure that the entire root ball is enclosed in plastic so the only way the water can get out is really through transpiration in the plant and we want to do that to all of our plants we're going to record the mass of each of those plants and so write that Mass on there this looks like 96.61 grams one for our initial mass for that plant just going to put that aside do the same thing with the other three plants this one is 92.36 put that one aside 74.49 89.40 now that we've got all of our plants and our little bags what we want to do next is we're going to put them in the different conditions it does recommend removing the flowers but you don't have to just consider that if the flowers fall off you want to also mask those as well in the end makes it easier to remove the flowers but you don't have to remove the flowers so one of them is going to stay in our room conditions so we'll just use our little one as our room and that's our control one of them is going to go in the light one of them is going to go in an area with a fan and then one of them is going to go into an area with mist and how we do the Mist is we take a large bag so we've got a two gallon bag and what you want to do is we're going to use a spray bottle and we're just going to mist in the bag and that's going to stimulate humid environments and we're going to put one of those in there pretty much room as possible standing up in there and that simulates a humid environment and so that will be left on our counter with our control standing up just wanted to clean it up against the sink and then one is going to go over into our area with a fan and one is going to go into our area with the light card so let's go grab these and take them over to where they need to go them and so over here we have an area in our fume Hood it has a little fan and the fume hood is going and so we're going to let that sit in there with the fan and then we're going to carry the last one over here to our light cart and we're gonna place that one in the light card and then they will sit there until our next session where we will record our data in two days and then our class after that we will record our final data and we'll measure change in mass over time we'll also collect the class data and we will use class data averages to calculate our transpiration rate okay we're going to pick up our plants from the light cart and from the wind by the fan and we're going to carry them back over to the balance and we're going to measure the change after it's been in those various conditions for couple of days so we're measuring now two days later because this was our light so we're going to put that on the balance and now our mass is 85.55 grams 85.55 grams our fan has gone down to 83.26 and then we have our room our control and that's at 72.16 one that's in our plastic bag remove that from the bag that off a little bit there's now moisture on the outside we don't want to mess that kind of drying the bag a little bit how much that is so 96.97 foreign looks like it went up slightly but that's probably because there's water on the string and on the bag so it really shouldn't go up at all but slightly because bag has gotten wet from the moisture in the bag so we're gonna put those things back again so we're going to leave that on the counter these two back to the wind and back to the light cart and we'll let them sit again until next class period so this one goes back the fan and then this one goes back in our light cart and we'll let that sit again until next class again we're going to pick up our samples one from the light we're gonna grab the one from the fan and we're gonna carry these over to the balance and we're going to measure the change in mass again so we'll do the light first like it went down to 62.22 grams and that was in the Wind went down to 64.16 room condition here so here is our room and that and that went down to 67.27 foreign that was in the Mist dry that off we've got some flowers that seem to be falling off so we do need to keep those in there we want to get the moisture off all these little flower parts I might have gotten a little bit broken one flower two flowers still in the bag keep that mess in there as well and that's at 96.93 90. 96.9 [Music] 3 grams and those masses seem to actually I've gone up slightly but that's just because it's probably picked up moisture from the bag so you can see little water drops on the bag and things like that so essentially it hasn't changed in Mass the whole time but the other ones we can definitely tell have decreased in Mass so what we really want to do is we want to see which one has the greatest change which one lost the most Mass and so we can use our data we'll also collect class data and we will graph the average of the class data and see which environmental Factor has the greatest effect on transpiration and so we will can compare all of that to our control which is our room condition plant so we will look at the class data and then analyze our data from there