in this video we will prepare platelet rich plasma with the ease of pure two platelet concentrating system to begin remove the Red Cap from the needless valve port of the separator device swab the port with sterile alcohol before and after accessing with a sterile syringe or cap fill a 30ml sterile syringe with 3 ml of citrate anti-coagulant then draw 27 mL of blood from the patient filling the syringe to 30 ml attach the 30ml blood syringe to the needleless port of the separator device and slowly inject the anti-coagulated blood use the counterbalance device to counterbalance with equal volume Place both devices in the centrifuge rotor at opposite ends follow the protocol settings provided in the pure 2 ifu insert close the centrifuge lid and press start after centrifugation the platelet plasma suspension or PPS will be separated above the red blood cell layer attach a sterile syringe to the top port and aspirate the platelet plasma suspension while holding the concentrator device in an upright position transfer the platelet plasma suspension through the bottom [Music] Port as a mandatory step always attach the clear non-vented cap to the bottom Port prior to centrifugation counterbalance with equal volume be sure to place the clear non-vented cap to the bottom Port of the counterbalance device as well and place back into the centrifuge Rotor at opposite ends follow the protocol settings provided in the pure 2 ifu insert close the centrifuge lid and press start after centrifugation the platelet Buffy coat layer will be clearly visible at the bottom of the device attach a sterile syringe to the needleless port and aspirate plasma leaving 3 to 4 ml or the desired amount in the [Music] device resuspend the platelet Buffy coat back into the plasma by rocking and swirling the device back and forth do this until all of the platelets have been resuspended into the plasma solution and is no longer visible at the bottom of the device attach the 12 m M syringe to the needless port and tilt the device to aspirate The PRP this concludes our processing video for mite's Pure 2 processing system [Music]