Frog Dissection and Nerve Stimulation Procedure

Preparation

• Set Up: Place a pithed frog on a dissection board with its posterior surface facing upwards.

Skin Removal

• Cut Open Skin: Use scissors to cut open the skin of the frog, starting from the abdomen and continuing down to the lower extremities.
• Remove Skin: Carefully remove the skin to expose the underlying structures.

Muscle Exposure

• Cut Thigh Muscles: Use a scalpel to cut through the thigh muscles to expose the sciatic nerve.
• Pin Muscles: Pin the muscles down on either side to keep the area stable for further work.

Nerve Isolation

• Free the Nerve: Use a seeker to carefully free the sciatic nerve from the surrounding tissue.
• Insert Forceps: Place a pair of forceps under the nerve to assist in handling it.
• Moisten Nerve: Apply a few drops of Ringer's solution to keep the nerve and muscle moist.

Muscle Stabilization

• Fix the Muscle: Pin the muscle at the knee to stabilize its position for the stimulation process.

Nerve Stimulation

• Apply Voltage:
  • First, apply 6 volts to stimulate the nerve and observe any changes in the calf muscle.
  • Next, apply 10 volts to stimulate the nerve again and observe changes in the calf muscle.