[Music] place a pithed frog and a dissection board with its posterior surface facing upwards cut open the skin of the frog using a pair of scissors and remove the skin from the abdomen down the lower extremities cut the thigh muscles with the scalpel to expose the sciatic nerve [Music] pin the muscles down on either side [Music] then use a seeker to free the nerve from surrounding tissue insert a pair of forceps under the nerve moisten the nerve and the muscle with a few drops of ringer's solution finally fix the muscle at the knee with a pin to stabilize its position after fixing the muscle at the knee apply six volts to stimulate the nerve observe any changes of the calf muscle then apply 10 volts to stimulate the nerve again observe any changes of the calf muscle